| Popis: |
Gas-liquid chromatography methpods were developed for the determination of transformation products and unaltered substrate in mirobial N -dealkylation studies with drug molecules. the microorganism used was the fungus Cunninghemella echinulata , and the drugs employed included morphine alkaloids, various N -substituted 6,7-benzomorphans, and diazepam, a member of the benzodiazepine group of drugs. Assays based on the GLC system utilizing a non-polar SE-30 stationary phase and employing a derivatization step prior to chromatography were devised. The development of these methods facilitated quantitative studies on microbial N -dealkylation and the relationship between substrate structure and microbial transformation activity. |
