Proposition of a Simplified Protocol and New Parameter Introduction in NMRI Mice Anhedonia Induction
| Autor: | Damir Bogdan, Elsa Fedrigolli, Pavle Banović, Dusan Lalosevic |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
business.industry
05 social sciences Anhedonia General Medicine behavioral disciplines and activities 03 medical and health sciences 0302 clinical medicine Nmri mice medicine 0501 psychology and cognitive sciences 050102 behavioral science & comparative psychology medicine.symptom business Neuroscience psychological phenomena and processes 030217 neurology & neurosurgery |
| Zdroj: | Serbian Journal of Experimental and Clinical Research. |
| ISSN: | 2335-075X 1820-8665 |
| DOI: | 10.2478/sjecr-2020-0021 |
| Popis: | Introduction: A broad spectrum of research involving stress and stress protocols has long proven that a point of anhedonia, social defeat and learned helplessness can be achieved and observed – with anhedonia being a clinical symptom of Anxiety, Depression and Bipolar Affective Disorder. Aim: The aim of this study is the development of a simplified protocol for anhedonia induction in NMRI male mice in order to shorten the period of mice suffering and decrease complexity of the procedure for other researchers and introduction of new parameter in order to achieve better standardization of results. Materials and Methods: 21 male NMRI mice were introduced to 2 different stress protocols (one found in literature and one simplified) where cognitive- behavioral status was tested using the Sucrose Preference Test, Open Field Test, Grooming Pattern and histological examination of adrenal glands, and to propose a new protocol for fellow researchers. Results: Results observed include the successful induction of anhedonia proven by Sucrose Preference Tests, Barbering effect and microhemorrhage of the adrenal glands. Simplified protocol showed superiority compared to the one found in literature. Conclusion: Simplified protocol showed higher efficiency and reduced amount of work during testing phase. Introduction of NMI as a new parameter during behavioral evaluation resulted in better standardization of measured SPT values that incorporates common knowledge of mammal physiology. A big developmental step was the introduction of the Normalised Mass Index to even out mass fluctuations and differences in basal metabolism, which we recommend to other researchers and institutions. |
| Databáze: | OpenAIRE |
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