| Autor: |
Ku Youn Baik, Il Hwan Seo, Myoung-Won Chae, Eun Ha Choi, Chae Bok Lee, Han Sup Uhm, Jae Woo Park |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Clinical Plasma Medicine. 9:31 |
| ISSN: |
2212-8166 |
| Popis: |
Tumors can form tumor microenvironment which educates tumor associated macrophages (TAMs) to change their identity from killing M1 stage to repair M2 stage. M2-like TAMs not only support tumor growth and migration but also prevent the action of other immune cells which attack tumors. Those functions of macrophages are mediated by many factors. Since nitric oxide (NO) is known as a key regulator of macrophage activation, we focused on NO-containing plasma activated water (NO-PAW) as a possible adjuvant to reorganize TAMs. In this study, we found that NO-PAW up-regulates M1 type macrophage activation and down-regulates M2 type macrophage characteristics. Translational and transcriptional levels of M1- or M2-related markers were examined. In addition, we observed that NO-PAW enhanced the killing potential of M2-type macrophages on melanoma cells in vitro and in vivo. In conclusion, our study suggests that NO-PAW induces and maintains activation of macrophages under tumor microenvironment. Download : Download high-res image (324KB) Download : Download full-size image Figure 1 (a) NO-PAW was generated by using microwave-plasma device with N2 and O2 gases. (b) NO-PAW activated macrophages to have high potential to kill melanoma cells. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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