Calcium alginate entrapment of Aspergillus nidulans IMPP-0785 laccase for enhanced enzyme catalytic ability, thermost ability and dye-decolorization

Autor:	Rao Wali Muhammad, Malik Muhammad Yousaf, Hafiz Muhammad Zia Ullah Ghazali, Mumtaz Hussain, Muhammad K. Majeed, Bashir Ahmad, Muhammad Jahangir Shah
Rok vydání:	2018
Předmět:	Laccase chemistry.chemical_classification Entrapment chemistry.chemical_compound Enzyme Calcium alginate Biochemistry chemistry biology Aspergillus nidulans Plant Science biology.organism_classification Catalysis
Zdroj:	Bangladesh Journal of Botany. 47:903-909
ISSN:	2079-9926 0253-5416
Popis:	Production of laccase using a solid-state culture of Aspergillus nidulans IMPP-0785 was optimized using barley bran. Aspergillus nidulans IMPP-0785 laccase optimum activity was observed with filtrate (29.15 U/g) as compared to supernatant and unfiltered. Free laccase and supernatant gave best results at 30.24 and 25.95 U/g, respectively for 20 min incubation time. The entrapped spores of A. nidulans IMPP-0785 showed maximal activity (16.12 U/g) at incubation time for 60 min. Immobilized laccase resulted optimal activity (29.24 U/g) for 25 min of incubation. Enzyme showed higher thermo stability of 34.28 U/g when incubated for 35 min.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a6b49c83460333c318e8c1030425c97c https://doi.org/10.3329/bjb.v47i4.47380 Zobrazit plný text záznamu