Interaction of mammalian cell culture broth with adsorbents in expanded bed adsorption of monoclonal antibodies

Autor:	D. Lütkemeyer, M.-R. Kula, Jörg Thömmes, Markus Halfar, N. Ameskamp, J. Feuser
Rok vydání:	1999
Předmět:	Adsorption Chromatography Affinity chromatography Chemistry Cell culture Expanded bed adsorption Protein purification Ion chromatography Bioengineering Saturation (chemistry) Ion-exchange resin Applied Microbiology and Biotechnology Biochemistry
Zdroj:	Process Biochemistry. 34:159-165
ISSN:	1359-5113
DOI:	10.1016/s0032-9592(98)00083-1
Popis:	The interaction of a mammalian cell culture broth with two commercially available adsorbents for the use in expanded bed adsorption (EBA) has been studied. A cation exchange resin (Streamline SP) and an affinity adsorbent (Streamline rProtein A) were compared with regard to adsorption of hybridoma cells during sample application as well as potential cell damage. The results showed that hybridoma cells interact significantly with an expanded bed of cation exchange adsorbents but not with the Protein A adsorbent. After application of 17–20 sedimented bed volumes a saturation of the Streamline SP resin with cells was noted. With both adsorbents no measurable cell damage was found and IgG1 was recovered in approximately 95% yield. The capacity for IgG1 adsorption at 3% breakthrough was 2.7 mg IgG1/ml Streamline rProtein A at a constant fluid velocity of 380 cm/h and 1.0 mg IgGl/ml Streamline SP at 215–240 cm/h fluid velocity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a6a3632692d58edb4eb464691da3ed13 https://doi.org/10.1016/s0032-9592(98)00083-1 Zobrazit plný text záznamu Full Text from ScienceDirect