Effect of topical retinoic acid on the interleukin Iα and β immunoreactive pool in normal human epidermis

Autor: D. Gumowski‐Sunek, L. Didierjean, D.Chatellard Gruaz, J.-H. Saurat
Rok vydání: 1990
Předmět:
Zdroj: British Journal of Dermatology. 123:283-289
ISSN: 1365-2133
0007-0963
DOI: 10.1111/j.1365-2133.1990.tb06286.x
Popis: The topical application of 0.1% retinoic acid (RA) on human skin over a period of 4 days, whether or not under occlusion, did not increase either IL-1 alpha or beta immunoreactivity as determined by a sensitive enzymoimmunoassay. No down modulation was seen following the application of a potent topical corticosteroid. Occlusion increased the yield of IL-1 beta immunoreactivity. Immunoblot patterns of epidermal extracts revealed both the mature form of IL-1 (17 kDa) and the precursor (36 kDa) and were identical in amounts whether the specimens were from controls or from RA- or corticosteroid-treated skin. There was a slight modification in the pattern of high molecular weight proteins (52 kDa) probed by the anti-IL-1 alpha and beta sera. It appears that the IL-1 epidermal immunoreactive pools are barely amenable to modulation because they represent a storage form linked to end-stages of keratinocyte differentiation.
Databáze: OpenAIRE
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