Electrochemical determination of prolintane in pharmaceutical formulations and in human urine

Autor: Lucas Hernández, Esperanza Bermejo, Antonio Zapardiel, A.G. Espartero, José A. Pérez-López
Rok vydání: 1997
Předmět:
Zdroj: Electroanalysis. 9:416-421
ISSN: 1521-4109
1040-0397
Popis: The oxidative behavior of 1-[1-(phenylmethyl)butyl]pyrrolidine, prolintane, was studied at a glassy carbon electrode using linear-sweep and differential-pulse voltammetry. The oxidation process was shown to be irreversible using 0.04 M Britton–Robinson buffer and was diffusion-adsorption controlled. Two voltammetric methods were developed for the determination of prolintane using different techniques: linear-sweep and differential-pulse voltammetry. The peak current varied linearly with prolintane concentrations in the range of 1.0 × 10−5 −2.5 × 10−4 M, with a detection limit of 8.5 × 10−6 and 4.0 × 10−6 M, and with relative standard deviations of 2.1 % and 3.1 %, respectively. The methods were applied to commercial preparations, giving relative errors less than 3.1 % and relative standard deviations lower than 4.8 % (n = 10). Determination of prolintane (down to the 8.5 × 10−8 M level) can be performed by using a preconcentration step prior to the determination by differential-pulse voltammetry in 0.04 M Britton–Robinson buffer (pH 8.0) with preconcentration potential of 0.0 V. The detection limit was found to be 6.2 × 10−8 M (4 min preconcentration) and the relative standard deviation for 2.5 × 10−7 M prolintane (n = 5) was 4.6 %. Applicability to human urine analysis is illustrated (recovery 98 ± 2 %). Standard additions method can be used to determine prolintane in real samples of urine.
Databáze: OpenAIRE
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