Production of Nanobiocontrol Agent in Molasses and Tofu Liquid Waste Media by Streptomyces sp. TT10
| Autor: | Sri Andayani Desak Gede, Toni Kristiantoro, Hermida Putu I. Dewa, Desak Gede Tirta Andini, Novik Nur Hidayat |
|---|---|
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | IOP Conference Series: Materials Science and Engineering. 553:012039 |
| ISSN: | 1757-899X 1757-8981 |
| DOI: | 10.1088/1757-899x/553/1/012039 |
| Popis: | The objective of this study has processed the waste of agriculture into high economic value materials using fermentation technology by Streptomyces sp. TT 10 in the form of new materials in Nano size. In this study, anti-phytopathogenic agents would be produced using fermentation technology in the form of antifungal that fought fungi that often infected strawberries. The research series consisted of the fermentation process for seven days, 30°C, pH 7 on molasses and tofu liquid waste medium. The broth of fermentation was extracted using ethyl acetate. Concentrated ethyl acetate extracts were ground using a shaker mill PPF-UG to obtain the size up to 100 nm. Furthermore, Nano sizes were characterized using (particle size analyzer) PSA and (scanning electron microscope) SEM. The anti-phytopathogenic agent was analyzed by agar diffusion and microdilution. The results showed that activity of ethyl acetate extract of Streptomyces sp. TT10 was increased up to 80% after milling process, against pathogenic strawberry that is Penicillium sp., Aspergillus sp., Rhodotorula sp., Kluyveromyces sp., Candida krusei, Candida pseudotropicalis and Fusarium oxysporum with inhibitory diameter (mm), minimum inhibition concentrations (MIC) (ppm) and the minimum fungicidal concentration (MFC) (ppm) value (%) are respectively (82.85:50;50), (81.29;50;50), (82.85;50;50), (83.87;50;50), (74.07;50;50), (79.95;50;50), and (83.33; 50; 50). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|