Immunoassays based on Pt nanoparticles with peroxidase activity
| Autor: | Jong Min Park, Min Jung Kang, Jae Chul Pyun, Ha Wook Jung, Hyung-Seok Kim, Young Wook Chang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Thermogravimetric analysis
Bioengineering Nanotechnology 02 engineering and technology 010402 general chemistry Platinum nanoparticles 01 natural sciences Luminol chemistry.chemical_compound Materials Chemistry medicine Electrical and Electronic Engineering medicine.diagnostic_test biology Chromogenic Substrate (chemistry) 021001 nanoscience & nanotechnology Condensed Matter Physics 0104 chemical sciences chemistry Immunoassay biology.protein 0210 nano-technology Luminescence Peroxidase Nuclear chemistry |
| Zdroj: | International Journal of Nanotechnology. 13:402 |
| ISSN: | 1741-8151 1475-7435 |
| Popis: | In this work, Pt nanoparticles were synthesised according to the citrate reduction process. The physical properties of these Pt nanoparticles were characterised using dynamic laser scattering (DLS), zeta-potential analysis, thermal gravimetric analysis (TGA), and TEM. The peroxidase activity of the Pt nanoparticles was also analysed using a chromogenic substrate (TMB) and a luminescent probe (luminol). For application to immunoassays, Pt nanoparticles were coupled with antibodies via electrostatic interaction, and the coupling conditions were optimised at different pH conditions. Finally, the applicability of Pt nanoparticles coupled with anti-hCG (human chorionic gonadotropin) antibodies was demonstrated for the immunoassay of hCG for the medical diagnosis of pregnancy. |
| Databáze: | OpenAIRE |
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