Metacaspase MC1 enhances aluminum-induced programmed cell death of root tip cells in Peanut

Autor: Shaochang Yao, Long-Fei He, Jie Zhan, Shuzhen Luo, Dong Xiao, Ai-Qin Wang, Wei-Jiao Xiong, Chunliu Pan
Rok vydání: 2020
Předmět:
Zdroj: Plant and Soil. 448:479-494
ISSN: 1573-5036
0032-079X
DOI: 10.1007/s11104-020-04448-w
Popis: Metacaspases are cysteine-dependent proteases, which play essential roles in programmed cell death (PCD), and caspase-3-like protease is the crucial executioner. However, its response mechanism to aluminum (Al)-induced PCD is still elusive. Here, the type I metacaspase gene in peanut (Arachis hypoganea L.), AhMC1, was cloned from the Al-sensitive cultivar ZH2. Physiological and biochemical methods, as well as gene expression analyses, were employed to explore its function in Al-induced PCD in peanut root tips. AhMC1 had a 1068-bp open reading frame, encoding a peptide of 355 amino acids, and the purified protein exhibited a high caspase-3-like protease activity. Its expression levels in different tissues of peanut varieties ZH2 and 99–1507 (Al-tolerant) varied under Al-stress conditions. The subcellular localization indicated that AhMC1 was transferred from mitochondria into the cytoplasm. Furthermore, overexpressing AhMC1 reduced the resistance to Al stress. Sense transgenic plants showed a low relative root growth rate, and reduced superoxide dismutase, peroxidase, and catalase activities, compared with wild-type and antisense transgenic plants under Al-stress conditions, but had a high root-cell death rate, and increased Al and maleic dialdehyde contents. The data suggest that metacaspase AhMC1 is a positive factor in Al-induced PCD in peanut root tips.
Databáze: OpenAIRE
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