Structure of amylase-binding protein A of Streptococcus gordonii : A potential receptor for human salivary α-amylase enzyme

Autor:	Narayanan Ramasubbu, Ashish Sethi, Biswaranjan Mohanty, Paul R. Gooley
Rok vydání:	2015
Předmět:	chemistry.chemical_classification Binding protein Dental pellicle Streptococcus gordonii Biology biology.organism_classification Biochemistry Microbiology stomatognathic diseases Enzyme Protein structure chemistry biology.protein Salivary alpha-Amylases Amylase Protein A Molecular Biology
Zdroj:	Protein Science. 24:1013-1018
ISSN:	0961-8368
DOI:	10.1002/pro.2671
Popis:	Amylase-binding protein A (AbpA) of a number of oral streptococci is essential for the colonization of the dental pellicle. We have determined the solution structure of residues 24–195 of AbpA of Streptococcus gordonii and show a well-defined core of five helices in the region of 45–115 and 135–145. 13Cα/β chemical shift and heteronuclear 15N-{1H} NOE data are consistent with this fold and that the remainder of the protein is unstructured. The structure will inform future molecular experiments in defining the mechanism of human salivary α-amylase binding and biofilm formation by streptococci.
Databáze:	OpenAIRE
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