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Background Qingfei Tongluo formular (QT) is a homemade agent for the Mycoplasma pneumoniae pneumonia (MPP) treatment developed by pediatrics of Shanghai Longhua hospital, which combining with AZM(azithromycin) has better clinical curative effect. This study aims at exploring the chemical components and clarifying the possible mechanism involved in. Methods LC-MS was used for chemical analysis. The mycoplasma pneumoniae infected mice were treated with AZM and QT (46.25 and 92.5 mg/g) for 3 days. Polymorphonuclear neutrophils (PMNs) and monocytes in bronchoalveolar lavage fluid (BALF) were measured by haemacytometer. IL-6, IL-10, IL-1β and IL-13 in BALF were identified by ELISA assay. HE staining was employed for histological examination. TLR4, COX-2 and NF-κB expressions were assessed by western blot.Student‘s t test was used to compared the differences between two groups, while one-way analysis of variance was used when more than two groups were compared. P |
