Qy-Level Structure and Dynamics of Solubilized Light-Harvesting Complex II of Green Plants: Pressure and Hole Burning Studies
| Autor: | G. Renger, J. Voigt, M. Ratsep, Ryszard Jankowiak, K.-D. Irrgang, J. || Pieper, Gerald J. Small |
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| Rok vydání: | 1999 |
| Předmět: | |
| Zdroj: | The Journal of Physical Chemistry A. 103:2412-2421 |
| ISSN: | 1520-5215 1089-5639 |
| DOI: | 10.1021/jp983957l |
| Popis: | Nonphotochemical hole burning and pressure-dependent absorption and hole-burning results are presented for the isolated (disaggregated) chlorophyll a/b light-harvesting II trimer antenna complex of green plants. Analysis of the 4.2 K burn-fluence dependent hole spectra and zero-phonon hole action spectra indicates that the three lowest energy states (Qy) lie at 677.1, 678.4 and 679.8 nm. Their combined absorption intensity is equivalent to that of three Chl a molecules. The inhomogeneous broadening of their absorption bands is 70 cm-1. It is argued that these states, separated by 30 cm-1, are associated with the lowest energy state of the trimer subunit with the 30 cm-1 separations due to the indigenous structural heterogeneity of protein complexes. The linear electron−phonon coupling of the 679.8 nm state is weak and characterized, in part, by a mean phonon frequency of ωm = 18 cm-1 and Huang−Rhys factor of Sm = 0.8, values which yield the correct Stokes shift for fluorescence from the 679.8 nm state at ... |
| Databáze: | OpenAIRE |
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