A Low-Volume, Parallel Copper-Bicinchoninic Acid (BCA) Assay for Glycoside Hydrolases
| Autor: | Jathavan Asohan, Harry Brumer, Gregory Arnal, Mohamed A. Attia |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine chemistry.chemical_classification Kinetics chemistry.chemical_element Polysaccharide 01 natural sciences Copper law.invention Reducing sugar Low volume 03 medical and health sciences 030104 developmental biology chemistry Biochemistry law Bicinchoninic acid assay Glycoside hydrolase Polymerase chain reaction 010606 plant biology & botany |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493968985 |
| DOI: | 10.1007/978-1-4939-6899-2_1 |
| Popis: | The quantitation of liberated reducing sugars by the copper-bicinchoninic acid (BCA) assay provides a highly sensitive method for the measurement of glycoside hydrolase (GH) activity, particularly on soluble polysaccharide substrates. Here, we describe a straightforward method adapted to low-volume polymerase chain reaction (PCR) tubes which enables the rapid, parallel determination of GH kinetics in applications ranging from initial activity screening and assay optimization, to precise Michaelis-Menten analysis. |
| Databáze: | OpenAIRE |
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