| Autor: |
Abdelhameed Moussa, Stefano Nones, Patrizia Elena Vannucchi, Gul-i-Rayna Shahzad, Jessica Dittmer, Erika Corretto, Martin Schebeck, Massimo Faccoli, Andrea Battisti, Christian Stauffer, Hannes Schuler |
| Rok vydání: |
2023 |
| Popis: |
The European spruce bark beetleIps typographus(L.) is a pest causing severe damage to Norway spruce-dominated forests in Europe. Microorganisms play an essential role in the species life history, including nutrition, fitness as well as in overcoming host defenses. Here, we performed high-throughput 16S rRNA metabarcoding ofI. typographusacross different populations in Europe. We investigated four postglacial refugial areas in Europe and focused specifically on a current bark beetle hot spot in the Dolomites where we compared populations with different epidemiological phases (outbreaking vs. non-outbreaking) and across different seasons (pre-overwintering vs. overwintering). Our results show that the bacterial community structure varied among populations from the refugial areas and geographic regions within the Dolomites. We found a significant difference in the bacterial community between pre-overwintering and overwintering individuals, highlighting a potential role of the microbiome inI. typographusoverwintering but we did not find differences between epidemic and endemic populations. The generaErwiniaandPseudoxanthomonas– previously reported for their role in nutrition and protection from conifer defense compounds – were present in every individual across all populations, suggesting that these taxa form the bacterial core community ofI. typographus. Furthermore, several additional bacterial taxa occurred in all populations, but with variable frequencies within and between individuals. This study highlights a complex interaction of various bacterial taxa across different regions and ecological phases ofI. typographuspopulations and provides new insights into the role of microorganisms in the biology of this important pest species. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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