246 INFLUENCE OF BREED AND SEASON ON IN VITRO EMBRYO PRODUCTION
| Autor: | B. Bernal, A. Tribulo, J. Revol, H. Tribulo, J. M. Oviedo, Gabriel A. Bó, G. Moreria |
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| Rok vydání: | 2016 |
| Předmět: |
Bonsmara
biology business.industry Brahman Embryo culture Reproductive technology Beef cattle biology.organism_classification humanities Breed Biotechnology Endocrinology Animal science Reproductive Medicine Reproductive biology Genetics Animal Science and Zoology business Molecular Biology Dairy cattle Developmental Biology |
| Zdroj: | Reproduction, Fertility and Development. 28:255 |
| ISSN: | 1031-3613 |
| DOI: | 10.1071/rdv28n2ab246 |
| Popis: | A retrospective analysis of in vitro production (IVP) data was done to determine the influence of breed and season on the production of viable oocytes and embryos. Cumulus‐oocyte complexes (COC) were obtained from 1946 ultrasound-guided follicle aspiration (ovum pickup) sessions performed at random stages of the oestrous cycle without superstimulation in Bos taurus and Bos indicus donors in commercial IVP in Argentina. Frozen-thawed conventional semen was used in beef cattle and conventional (n = 139) and sexed-selected (n = 481) semen in dairy cattle. The COC were classified, matured in B-199 medium, fertilized in IVF-SOF medium (Day 0), and cultured in SOF medium supplemented with 0.4% BSA under oil at 38.8°C, 5.5% CO2, and saturated humidity for 7 days. The number of viable COC and transferable embryos in each breed and season were compared by ANOVA and means were compared by Fisher’s Least Significant Difference test. Proportions were first transformed by arcsin and then analysed by ANOVA. To simplify the interpretation of the results, breeds were grouped as follows: dairy Bos taurus (Holstein, n = 620), beef Bos taurus (Angus and Bonsmara, n = 229), Bos taurus × Bos indicus (Brangus and Braford, n = 1045), and Bos indicus (Brahman, n = 52). There was no interaction between breed and season for any of the end points analysed (P > 0.1). Mean (± standard error of the mean) numbers of viable COC and transferable embryos were higher (P |
| Databáze: | OpenAIRE |
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