Detection of lymphocystis disease virus (LCDV) in olive flounder (Paralichthys olivaceus) using efficient extraction and concentration methods

Autor:	Jae-Hak Moon, Chae Hong Lim, Myung-Joo Oh, Jong-Oh Kim, Duwoon Kim, Seok-Ryel Kim, Jeong-Young Cho, Wi-Sik Kim, Jong-Bang Eun, Byeong-Mun Jeong, Myoung-Ae Park
Rok vydání:	2010
Předmět:	Detection limit Tris biology Lymphocystivirus Paralichthys Lymphocystis biology.organism_classification Applied Microbiology and Biotechnology DNA extraction Molecular biology Olive flounder Virus chemistry.chemical_compound chemistry Food Science Biotechnology
Zdroj:	Food Science and Biotechnology. 19:1693-1696
ISSN:	2092-6456 1226-7708
DOI:	10.1007/s10068-010-0241-5
Popis:	Efficiency of extraction and concentration methods for the detection of the major capsid protein gene of lymphocystis disease virus from different tissues of olive flounders (Paralichthys olivaceus) was tested. Tris elution buffer showed a 100 fold higher polymerase chain reaction (PCR) detection limit than TE elution buffer in the virus extraction step from skin tissues. Using the TRPD (Tris elution buffer, polyethylene, and DNA extraction kit) procedure, we confirmed that skin tissues and lymphocystis cells of olive flounders had a detection limit of 10 �6 and 10 �7 PCR-U/µL, meaning that 10 6 and 10 7 fold dilutions of lymphocystis disease virus (LCDV) were PCR positive, respectively.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a6165edbbc0b73493b3b8f2ed9ca5b45 https://doi.org/10.1007/s10068-010-0241-5 Zobrazit plný text záznamu Full text from SpringerLink