| Popis: |
The aim of this study was to evaluate whether use of Aloysia triphylla essential oil (EOAT) (2 mL/kg feed) was able to prevent or reduce the plasma biomarkers of renal and hepatic damage in silver catfish Rhamdia quelen fed with ochratoxin-A (OTA)-contaminated diet. In this study, 126 juvenile silver catfish (six treatments with three repetitions, seven fish per repetition), as follow: control (basal diet, i.e., without EOAT and OTA), EOAT 2 mL/Kg feed, OTA 200 (200 µg OTA/kg feed), OTA 400 (400 µg OTA/kg feed), OTA 200 + EOAT 2 mL/Kg feed (200 µg OTA/kg feed + EOAT 2 mL/Kg feed) and OTA 400 + EOAT 2 mL/Kg feed ( 400 µg OTA/kg feed + EOAT 2 mL/Kg feed), that were fed during 32 days. On the last day of the experiment the animals were weighed and slaughtered, and blood was collected for processing and plasma was obtained for analysis of enzymes and metabolites that could indicate liver and kidney damage. Serum glutamic oxaloacetic transaminase (GOT) and glutamic oxaloacetic transaminase (GOT) were significantly higher in fish feed with OTA compared to control group, while no significant difference was observed between these groups regarding creatinine, protein and lactate levels. The use of EOAT 2 mL/Kg feed was able to prevent the increase on GPT levels elicited by OTA-diet, but not exerted significant effects on GOT levels. This result indicated that EOAT can be an alternative to prevent/reduce OTA-induced liver damage in silver catfish. |
