Carbohydrate metabolism in erythrocytes of copper deficient rats11Publication # 538 of the Bureau of Nutritional Sciences

Autor: Louise J. Plouffe, Brian J. Lampi, Stephen P. J. Brooks, W.M.N. Ratnayake, D. B. Black, Kevin A. Cockell, Brian Dawson, Bartholomeus Belonje
Rok vydání: 2003
Předmět:
Zdroj: The Journal of Nutritional Biochemistry. 14:648-655
ISSN: 0955-2863
DOI: 10.1016/j.jnutbio.2003.08.002
Popis: Dietary copper deficiency is known to adversely affect the circulatory system of fructose-fed rats. Part of the problem may lie in the effect of copper deficiency on intermediary metabolism. To test this, weanling male Long-Evans rats were fed for 4 or 8 weeks on sucrose-based diets containing low or adequate copper content. Copper deficient rats had significantly lower plasma and tissue copper as well as lower plasma copper, zinc-superoxide dismutase activity. Copper deficient rats also had a significantly higher heart:body weight ratio when compared to pair-fed controls. Direct measurement of glycolysis and pentose phosphate pathway flux in erythrocytes using 13 C NMR showed no differences in carbon flux from glucose or fructose to pyruvate but a significantly higher flux through the lactate dehydrogenase locus in copper deficient rats (approximately 1.3 times, average of glucose and glucose + fructose measurements). Copper-deficient animals had significantly higher erythrocyte concentrations of glucose, fructose, glyceraldehyde 3-phosphate and NAD + . Liver metabolite levels were also affected by copper deficiency being elevated in glycogen and fructose 1-phosphate content. The results show small changes in carbohydrate metabolism of copper deficient rats.
Databáze: OpenAIRE
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