High Specificity Binding of Lectins to Carbohydrate-Functionalized Fiber Bragg Gratings: A New Model for Biosensing Applications

Autor:	Matthew T. Hurley, Geunmin Ryu, Mario Dagenais, Philip DeShong
Rok vydání:	2010
Předmět:	Peanut agglutinin biology Chemistry business.industry Carbohydrate Fluorescence Glycome Atomic and Molecular Physics and Optics Optics Fiber Bragg grating biology.protein Biophysics Fiber Electrical and Electronic Engineering business Biosensor Conjugate
Zdroj:	IEEE Journal of Selected Topics in Quantum Electronics. 16:647-653
ISSN:	1558-4542 1077-260X
Popis:	The functionalization of an etched fiber Bragg grating was realized using a carbohydrate-siloxane conjugate. No fluorescent probes were used. Concanavalin A bound with high specificity to the glucose biosensor, but not to the lactose functionalized fiber. Conversely, peanut agglutinin bound to the lactose sensor with high specificity over its glucose counterpart. Quasi-monolayer selective binding of the lectins to the fiber sensor was inferred based on a theoretical analysis of the observed changes in the refractive index. Our results open the way to the use of unlabeled carbohydrate-based sensors for the study of the human glycome.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a5f63ecc3a73d906f452178bb4805b8e https://doi.org/10.1109/jstqe.2009.2032427 Zobrazit plný text záznamu