Neurons exhibitLyz2promoter activity in vivo: Implications for using LysM-Cre mice in myeloid cell research
| Autor: | Angela Schulz, Kerstin Immig, Jens Eilers, Johannes Hirrlinger, Johannes Orthgiess, Ingo Bechmann, Martin Gericke |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Myeloid Microglia Immunology Cell Gene targeting Biology Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure nervous system Gene Knockout Techniques Conditional gene knockout medicine Immunology and Allergy Homologous recombination 030217 neurology & neurosurgery Neuroinflammation |
| Zdroj: | European Journal of Immunology. 46:1529-1532 |
| ISSN: | 0014-2980 |
| DOI: | 10.1002/eji.201546108 |
| Popis: | To characterize LysM-Cre mediated gene targeting in mice, we crossed LysM-Cre mice to two independent reporter-mouse lines (tdTomato or YFP). Surprisingly, we found that more than 90% of cells with LysM-Cre mediated recombination in the brain were neurons, rather than myeloid cells, such as microglia. Hence, by using the LysM-Cre mouse line for conditional knockout approaches, a significant neuronal recombination needs to be considered. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text