GW24-e2258 Urotensin II is involved in myocardial fibrosis of diabetic cardiomyopathy in rats fed with fructose
| Autor: | Bo-Zhi Cai, Yong-Gang Zhang, Li-Biao Wu, Zi-Han Chen, Meng-yuan Chen, Qian-Qian Wang, Bao-Jun Yang, Xiao-Ou Zhou, Wei-Zhao Lin |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
business.industry Cardiac fibrosis Insulin medicine.medical_treatment Fructose medicine.disease Metformin chemistry.chemical_compound Endocrinology chemistry Internal medicine Diabetic cardiomyopathy Diabetes mellitus medicine Myocardial fibrosis Cardiology and Cardiovascular Medicine Urotensin-II business medicine.drug |
| Zdroj: | Heart. 99:A39.1-A39 |
| ISSN: | 1468-201X 1355-6037 |
| DOI: | 10.1136/heartjnl-2013-304613.102 |
| Popis: | Objectives The aim of this experiment was to study the significance of UII and its receptor UT, and endothelial- to- mesenchymal transition (EndMT) during development of myocardial fibrosis in a rat model of diabetic mellitus fed with fructose. Methods Male SD rats (200 g), were randomly divided into 4 groups (n = 6-11): Control group; Metformin group [(treated with metformin (300 mg/kg·d) by gavage (8th-15th week)]; Fructose group (treated with 10% fructose in drinking water for 15 weeks); Fructose + Metformin group [treated with both fructose and metformin]. The changes of fasting blood-glucose and serum insulin, HDL, Masson staining, were observed. UII and TGF-β1 content were measured by radioimmunoassay. UT, α-SMA, fibroblast specific protein-1 (FSP-1) and collagen-I immunoreactivity in heart were detected by immunohistochemistry. Results Fasting blood-glucose and insulin level in the fructose group were higher than that of control group and /or the metformin groups (both p Conclusions The results showed that both myocardial UII and UT expression are elevated, with the emergence of EndMT, during the development of cardiac fibrosis induced by diabetes mellitus in rats fed with fructose, indicating that UII may play an important role in diabetic myocardial fibrosis through EndMT. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|