The effect of pH and concentration on the surface tension of adsorbed layers of various insulin preparations
Autor: | F.Y.H. Lin, A. W. Neumann, Walter Zingg, Z. Policova, D.Y. Kwok |
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Rok vydání: | 1995 |
Předmět: |
chemistry.chemical_classification
Chromatography Chemistry Insulin medicine.medical_treatment Drop (liquid) INSULIN PREPARATIONS High resolution Surfaces and Interfaces General Medicine Drop shape Amino acid Surface tension Colloid and Surface Chemistry Adsorption medicine Physical and Theoretical Chemistry Biotechnology |
Zdroj: | Colloids and Surfaces B: Biointerfaces. 3:281-286 |
ISSN: | 0927-7765 |
DOI: | 10.1016/0927-7765(94)01145-u |
Popis: | A pendant drop arrangement is employed in conjunction with the axisymmetric drop shape analysis-profile (ADSA-P) technique to study the effect of concentration and pH on the surface tension of insulin layers adsorbed at the solution-air interface. Three different insulin preparations are used: human zinc-free insulin and two of its analogs, B9AspB27Glu and B10Asp, at three concentrations, 0.01, 0.1 and 1.0 mg ml −1 , and two pH levels, 3 and 7.5. ADSA-P is shown to permit high resolution between the insulin preparations which differ only by one or two amino acids in their primary structures. Furthermore, the effects of pH and concentration on the surface tensions of insulin solutions are examined. |
Databáze: | OpenAIRE |
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