Expression of insulin-like growth factor binding protein-4, insulin-like growth factor-I receptor, and insulin-like growth factor-I in the mouse uterus throughout the estrous cycle
| Autor: | Edith Markoff, Claudia Henemyre |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Estrous cycle
endocrine system medicine.medical_specialty Stromal cell urogenital system medicine.medical_treatment Growth factor Decidua Uterus Myometrium Cell Biology In situ hybridization Biology Insulin-like growth factor medicine.anatomical_structure Endocrinology Internal medicine Genetics medicine reproductive and urinary physiology hormones hormone substitutes and hormone antagonists Developmental Biology |
| Zdroj: | Molecular Reproduction and Development. 52:350-359 |
| ISSN: | 1098-2795 1040-452X |
| DOI: | 10.1002/(sici)1098-2795(199904)52:4<350::aid-mrd3>3.0.co;2-m |
| Popis: | Recent evidence suggests that a regulated insulin-like growth factor (IGF) system mediates the effects of estrogen, promoting the proliferation and differentiation of specific uterine cell types throughout the estrous cycle and during gestation in the rodent. Previous studies have shown that IGFs are differentially expressed in the mouse uterus during the periimplantation period. In the current study, we examined the expression of IGF binding protein-4 (IGFBP-4), IGF-I receptor (IGF-IR), and IGF-I in the mouse uterus throughout the estrous cycle. Ligand blot analysis was conducted on uterine homogenates using [125I]IGF-I. IGFBP-4 was detected in all uterine homogenates, varying in intensity throughout the estrous cycle. In situ hybridization studies at metestrus and diestrus demonstrated an intense IGFBP-4 mRNA signal in antimesometrial stromal cells between the luminal epithelium and the myometrium, but at proestrus and estrus, no IGFBP-4 signal was detected. No IGF-I mRNA was detected at any stage of the estrous cycle by in situ hybridization. However, by RT-PCR analysis, IGF-I mRNA was detected at all stages of the estrous cycle. RT-PCR analysis also showed IGF-IR mRNA throughout the estrous cycle. Using immunohistochemistry, IGF-IR immunostaining was detected throughout the estrous cycle and on days 2-7 of gestation, but was restricted to the glandular epithelium. These results suggest that uterine IGFBP-4 expression may not be dependent on uterine IGF-I expression. They also suggest that IGFBP-4 may play a role in uterine physiology independent of the inhibition of IGF-I action, and that IGF-IR is constitutively expressed in the mouse uterus. |
| Databáze: | OpenAIRE |
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