Pluronic F-68: an answer for shoot regeneration recalcitrance in microspore-derived Brassica napus embryos

Autor:	Philip A. Salisbury, Wayne Burton, Denise M. Barbulescu
Rok vydání:	2011
Předmět:	biology Brassica food and beverages Trifluralin Embryo Plant Science biology.organism_classification chemistry.chemical_compound Tissue culture chemistry Microspore Shoot Botany Doubled haploidy Zeatin Biotechnology
Zdroj:	In Vitro Cellular & Developmental Biology - Plant. 47:282-288
ISSN:	1475-2689 1054-5476
Popis:	Recalcitrance to tissue culture is observed in some genotypes of Brassica napus. Several studies have confirmed that Pluronic F-68 has growth-promoting effects on numerous tissue types. This work investigated the effect of the non-ionic surfactant Pluronic F-68 at four concentrations (0.1%, 0.25%, 0.5%, and 1% (w/v)) on the responsiveness of recalcitrant B. napus lines to tissue culture. Microspores from seven populations of B. napus were cultured on Nitsch and Nitsch medium with this compound. The embryos obtained were plated on solid B5 medium supplemented with zeatin for shoot induction. Pluronic F-68 had a highly significant effect on the proportion of shoot regeneration (P < 0.05) in some of the recalcitrant populations. However, no strong dose–response effect was observed. The estimated probability of a shoot occurring in the absence of Pluronic F-68 ranged from 0.04 to 0.31 depending on the genotype, while in the presence of Pluronic F-68, it ranged from 0.07 to 0.53, respectively.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a5d11885bed5d1c9fe3d1b1ee3944069 https://doi.org/10.1007/s11627-011-9353-8 Zobrazit plný text záznamu Plný text ve formátu PDF