| Popis: |
Publisher Summary This chapter presents recent studies on manifestations of delayed cellular hypersensitivity in vitro and in vivo . In laboratory, at least two different migration-inhibiting activities originating from the interaction of hypersensitive lymphocytes with the specific antigen were produced. One of these was produced with high doses of the specific antigen and possessed a direct macrophage inhibitory activity. It is probably identical with the migration inhibiting factor (MIF) of other studies. The results concerning the conditions of production, release, activity, chemical properties, etc., of this type of MIF are in general agreement with those of others. Cytological studies revealed that this MIF reduced the formation of pseudopodia, spreading, and mobility of macrophages in a monolayer. These events were frequently accompanied by changes in supravital staining. Additionally, MIF preparations were able to stimulate phagocytosis of Mycobacteria (BCG vaccine) by normal macrophages. The second migration-inhibiting activity found in experiments did not exhibit a direct effect on macrophage migration until the antigen was added to the test system. |
