Molecular genetic analysis reveals a putative bifunctional polyketide cyclase/dehydrase gene from Streptomycea coelicolor and Streptomyces violoceoruber, and a cyclase/O-methyltransferase from Streptomyces glaucescens

Autor:	Darrin P. Johnson, C. Richard Hutchinson, David H. Sherman, David A. Hopwood, Miguel Ángel Fernández-Moreno, Maureen J. Bibb, Eduardo Sebastian Martinez, Thomas J. Simpson, Francisco Malpartida
Rok vydání:	1991
Předmět:	biology Chemistry Organic Chemistry Streptomyces coelicolor biology.organism_classification Biochemistry Streptomyces Cyclase Actinorhodin Open reading frame Polyketide chemistry.chemical_compound Polyketide synthase Drug Discovery Gene cluster biology.protein
Zdroj:	Tetrahedron. 47:6029-6043
ISSN:	0040-4020
Popis:	Molecular genetic analysis of the actinorhodin gene cluster from Streptomyces coelicolor has revealed a putative bifunctional cyclase/dehydrase gene. Open reading frame (ORF) 4 from both the actinorhodin (act) and granaticin (gra) polyketide synthase (PKS) gene clusters were able to relieve the block in a mutant strain of S. coelicolor which produces mutactin. This compound is a shunt product ofthe actinorhodin biosynthetic pathway which results from an aberrant intramolecular aldol cyclization, and a failure to dehydrate the hydroxyl group at C-9. These results provide compelling evidence for the existence of a novel polyketide aldolase which specifies the correct cyclization of a complex oligoketide chain.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a5c1f4c35a140c044c0ee187b2bf1b5b https://doi.org/10.1016/s0040-4020(01)86494-2 Zobrazit plný text záznamu