CHOLINESTERASE IN THE BRAIN OF THE CECROPIA SILKMOTH DURING METAMORPHOSIS AND PUPAL DIAPAUSE
| Autor: | Bruce R. Locke, Daniel M. Eichenbaum, David G. Shappirio |
|---|---|
| Rok vydání: | 1967 |
| Předmět: |
chemistry.chemical_classification
medicine.medical_specialty media_common.quotation_subject fungi Biology Diapause Butyrylthiocholine Pupa Endocrinology Enzyme Biochemistry chemistry Internal medicine Acetylthiocholine medicine biology.protein Metamorphosis General Agricultural and Biological Sciences Esterase inhibitor Cholinesterase media_common |
| Zdroj: | The Biological Bulletin. 132:108-125 |
| ISSN: | 1939-8697 0006-3185 |
| DOI: | 10.2307/1539881 |
| Popis: | 1. The localization and properties of cholinesterase in the brain of the Cecropia silkmoth were investigated by histochemical and quantitative spectrophotometric methods utilizing acetylthiocholine as substrate.2. During pupal diapause, substantial activity was visualized in neuropile. At the outset of adult development, activity was also detected in adjacent regions occupied by neuronal or glial cell bodies.3. Only one form of cholinesterase was detected with certainty. On the basis of substrate-activity relationships for acetylthiocholine, propionylthiocholine and butyrylthiocholine, and on the basis of its sensitivity to certain selective esterase inhibitors, the enzyme has properties of an acetylcholinesterase.4. Substantial and essentially unchanging enzymatic activity was detected during pupation and most of pupal diapause, when the brain becomes endocrinologically inactive. However, an approximate doubling in activity was detected during storage of diapausing pupae at 6° C., apparently signalling t... |
| Databáze: | OpenAIRE |
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