Ultra-simple DNA extraction method for marker-assisted selection using microsatellite markers in rice

Autor:	Osamu Kamijima, Nonnatus S. Bautista, Tetsuya Yamada, Takashige Ishii, Nobuyuki Ikeda
Rok vydání:	2001
Předmět:	Chromatography Extraction (chemistry) Pipette food and beverages Plant Science Biology Marker-assisted selection DNA extraction Molecular biology chemistry.chemical_compound chemistry Microsatellite TE buffer Molecular Biology Genotyping DNA
Zdroj:	Plant Molecular Biology Reporter. 19:27-32
ISSN:	1572-9818 0735-9640
DOI:	10.1007/bf02824075
Popis:	We prevent an ultra-simple DNA extraction method for microsatellite analysis of rice. Each extraction requires only one microtube, one disposable pipette tip, TE buffer and few pieces (about 5 mm) of rice leaf tissue. This is sufficient for 200 PCR reactions. The extract can be kept in the freezer for long-term storage. Also, DNA can be extracted from 200–300 individuals in a few hours. These features enabled us to perform rapid largescale seedling genotyping required for marker-assisted selection. We have also examined the applicability of this method for other PCR-based markers: RAPDs, nuclear STS, chloroplast STS and chloroplast microsatellites.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a5a7c4cf0744da816269c6e87901f909 https://doi.org/10.1007/bf02824075 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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