Research Paper: Serum Proteomic Study of Women With Obsessive-Compulsive Disorder, Washing Subtype
| Autor: | Mona Zamanian-Azodi, Mohammad Mahboubi, Mohsen Hamidpour, Mostafa Rezaei-Tavirani, Mohammad Rostami-Nejad, Mohammad Kamran Derakhshan, Majid Rezaei Tavirani, Mostafa Hamdieh, Naser Nejadi |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Gene isoform medicine.medical_specialty Neurology Clinical neuroscience Inflammation Proteomics Fold change Pathophysiology 03 medical and health sciences Cellular and Molecular Neuroscience 030104 developmental biology 0302 clinical medicine Proteome Immunology medicine Neurology (clinical) medicine.symptom 030217 neurology & neurosurgery |
| Zdroj: | Basic and Clinical Neuroscience Journal. 9:337-346 |
| ISSN: | 2008-126X 2228-7442 |
| DOI: | 10.32598/bcn.9.5.337 |
| Popis: | Introduction Many genetic studies are conducted on Obsessive-Compulsive Disorder (OCD). however, a high-throughput examination of proteome profile of this severe disease has not been performed yet. Methods Here, the proteomic study of OCD patients' serum samples was conducted by the application of Two-Dimensional Electrophoresis (2DE) followed by Mass Spectrometry (MALDI-TOF-TOF). Results A total of 240 protein spots were detected and among them, five significant differentially expressed protein spots with the fold change of ≥1.5 were considered for further evaluations. These proteins include IGKC, GC, HPX, and two isoforms of HP. While IGKC and HP show down-regulation, GC and HPX indicate up-regulation. Moreover, a validation study of overall HP levels in patients' serum via nephelometric quantification confirmed the lower levels of this protein in the serum of OCD patients. Additionally, enrichment analysis and validation test revealed that inflammation is one of most dominant processes in OCD. Conclusion It is suggested that these candidate proteins and their underlying processes (especially, inflammation) may be linked to OCD pathophysiology and can promise a clinical use after extensive validation studies. |
| Databáze: | OpenAIRE |
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