Analysis of Streptomycin and Dihydrostreptomycin in Milk by Liquid Chromatography

Autor:	James D. MacNeil, Craig D. C. Salisbury, Geoff C Gerhardt
Rok vydání:	1994
Předmět:	Pharmacology Analyte Residue (complex analysis) Chromatography Elution Aminoglycoside Analytical Chemistry Whole milk chemistry.chemical_compound chemistry Streptomycin medicine Environmental Chemistry Perchloric acid Agronomy and Crop Science Food Science Dihydrostreptomycin medicine.drug
Zdroj:	Journal of AOAC INTERNATIONAL. 77:765-767
ISSN:	1944-7922 1060-3271
Popis:	A method developed for the determination of the aminoglycoside antibiotics streptomycin and dihydrostreptomycin in tissues was applied to the analysis of fluid milk. Samples are extracted with 3.6% perchloric acid, and then injected onto a trace enrichment column, from which they are eluted onto a reversed-phase analytical column. The analytes are detected by fluorescence following postcolumn derivatization with 1,2-naphthoquinone-4-sulfonic acid. Recovery of analytes was in the range of 50–65% for skim or partially defatted fluid milk, while recoveries for homogenized whole milk were lower. Limits of quantitation were 10 ppb for streptomycin and 20 ppb for dihydrostreptomycin.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a589e73cacd6cc8ebe3122a5c2ef6f70 https://doi.org/10.1093/jaoac/77.3.765 Zobrazit plný text záznamu