Blastema cell cycle in vitro and attempted restimulation of blastema cell cycling in denervated blastemata of the adult newt,Notophthalmus viridescens

Autor:	Morton Globus, Swani Vethamany-Globus, Bruce L. Tomlinson
Rok vydání:	1984
Předmět:	Denervation medicine.medical_specialty Cell General Medicine Cell cycle Biology In vitro Cell biology Endocrinology medicine.anatomical_structure In vivo Neurotrophic factors Internal medicine Notophthalmus viridescens medicine Animal Science and Zoology Blastema
Zdroj:	Journal of Experimental Zoology. 232:249-258
ISSN:	1097-010X 0022-104X
DOI:	10.1002/jez.1402320212
Popis:	The durations of the adult newt blastema cell-cycle phases were determined in vitro. In both ganglionated (G) and nonganglionated (nG) blastemata the total cell-cycle time was 62 hr; G1 + ½M, S, and G2 + ½M were 18, 37, and 7 hr, respectively. The lack of a significant difference in the durations of cell-cycle phases between G and nG explants suggests that nerve withdrawal does not influence the rate of progression through the cycle. A novel strategy was employed involving cell-cycle suppression (denervation in vivo) and continuous labeling followed by restimulation with nerves in vitro, in anticipation that nerve-dependent restricted cells will resume cycling. The data indicate that following prolonged denervation (48 hr), blastema cells may become refractory to neurotrophic factor.
Databáze:	OpenAIRE
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