Accumulation of Mucosal T Lymphocytes around Epithelial Cells after In vitro Infection with Cryptosporidium parvum
| Autor: | E J Brackett, C R Wyatt, Wendy J. Barrett |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Cellular immunity
Pathology medicine.medical_specialty T lymphocyte Biology biology.organism_classification Molecular biology Epithelium medicine.anatomical_structure Immune system Cryptosporidium parvum parasitic diseases medicine Intraepithelial lymphocyte Parasitology IL-2 receptor Ecology Evolution Behavior and Systematics CD8 |
| Zdroj: | The Journal of Parasitology. 85:765 |
| ISSN: | 0022-3395 |
| DOI: | 10.2307/3285762 |
| Popis: | We had previously shown that ileal intraepithelial lymphocytes isolated from calves with cryptosporidiosis include significantly increased numbers of CD8+ T lymphocytes and activated CD4+ cells. These increases could result from redistribution of resident mucosal lymphocytes or from homing of peripheral T cells to ileal mucosa. To determine whether resident mucosal lymphocytes can redistribute to Cryptosporidium parvum-infected epithelium, oocysts were inoculated in vitro onto ileum explants taken from 1-2-wk-old noninfected calves. After 24 hr of incubation, the explants were collected and frozen in liquid nitrogen. Immunohistochemical analysis of T-lymphocyte subpopulations was performed on sections, and labeled lymphocytes adjacent to villous epithelial cells were counted. Compared with uninoculated explants, there was a statistically significant increase in the number of CD8+ T lymphocytes per 100 epithelial cells in oocyst-inoculated tissue. In addition, there were increased numbers of CD4+ T cells and activated (CD25+) lymphocytes adjacent to C. parvum-infected epithelium. These results show that resident mucosal T lymphocytes can accumulate at the epithelium during C. parvum infection. |
| Databáze: | OpenAIRE |
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