Crystal structures and identification of novel Cd2+-specific DNA aptamer
| Autor: | Hehua Liu, Yanqing Gao, Johnsi Mathivanan, Zev Armour-Garb, Zhiwei Shao, Yixi Zhang, Xin Zhao, Qiyuan Shao, Weizhen Zhang, Jie Yang, Chulei Cao, Huili Li, Jia Sheng, Jianhua Gan |
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| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Nucleic Acids Research. 51:4625-4636 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gkad239 |
| Popis: | Cadmium (Cd) is one of the most toxic heavy metals. Exposure to Cd can impair the functions of the kidney, respiratory system, reproductive system and skeletal system. Cd2+-binding aptamers have been extensively utilized in the development of Cd2+-detecting devices; however, the underlying mechanisms remain elusive. This study reports four Cd2+-bound DNA aptamer structures, representing the only Cd2+-specific aptamer structures available to date. In all the structures, the Cd2+-binding loop (CBL-loop) adopts a compact, double-twisted conformation and the Cd2+ ion is mainly coordinated with the G9, C12 and G16 nucleotides. Moreover, T11 and A15 within the CBL-loop form one regular Watson–Crick pair and stabilize the conformation of G9. The conformation of G16 is stabilized by the G8–C18 pair of the stem. By folding and/or stabilizing the CBL-loop, the other four nucleotides of the CBL-loop also play important roles in Cd2+ binding. Similarly to the native sequence, crystal structures, circular dichroism spectrum and isothermal titration calorimetry analysis confirm that several variants of the aptamer can recognize Cd2+. This study not only reveals the underlying basis for the binding of Cd2+ ions with the aptamer, but also extends the sequence for the construction of novel metal–DNA complex. |
| Databáze: | OpenAIRE |
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