PredominantnifHtranscript phylotypes related toRhizobium rosettiformansin field-grown sugarcane plants and in Norway spruce
| Autor: | Thomas Hurek, Claudia Sofía Burbano, Yuan Liu, Barbara Reinhold-Hurek, Jesús Caballero-Mellado, Kim Leonie Rösner, Veronica Massena Reis |
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| Rok vydání: | 2011 |
| Předmět: |
Phylotype
biology food and beverages biochemical phenomena metabolism and nutrition biology.organism_classification Agricultural and Biological Sciences (miscellaneous) Symbiosis Botany Shoot Nitrogen fixation bacteria Diazotroph Ecology Evolution Behavior and Systematics Bradyrhizobium elkanii Temperature gradient gel electrophoresis Bacteria |
| Zdroj: | Environmental Microbiology Reports. 3:383-389 |
| ISSN: | 1758-2229 |
| DOI: | 10.1111/j.1758-2229.2010.00238.x |
| Popis: | Summary Although some sugarcane cultivars may benefit substantially from biological nitrogen fixation (BNF), the responsible bacteria have been not identified yet. Here, we examined the active diazotrophic bacterial community in sugarcane roots from Africa and America by reverse transcription (RT)-PCR using broad-range nifH-specific primers. Denaturing gradient gel electrophoresis (DGGE) profiles obtained from sugarcane showed a low diversity at all sample locations with one phylotype amounting up to 100% of the nifH transcripts. This major phylotype has 93.9–99.6% DNA identity to the partial nifH sequence from a strain affiliated with Rhizobium rosettiformans. In addition, nifH transcripts of this phylotype were also detected in spruce roots sampled in Germany, where they made up 91% of nifH transcripts detected. In contrast, in control soil or shoot samples two distinct nifH transcript sequences distantly related to nifH from Sulfurospirillum multivorans or Bradyrhizobium elkanii, respectively, were predominant. These results suggest that R. rosettiformans is involved in root-associated nitrogen fixation with sugarcane and spruce, plants that do not form root–nodule symbioses. |
| Databáze: | OpenAIRE |
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