| Autor: | V. M. Govorun, O. V. Smirnova, T A Akopian, V. V. Chelysheva, S.N. Borchsenius, Juan A. Ayala, A. N. Simankova, V. N. Lazarev, K. T. Momynaliev |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Cloning
biology macromolecular substances Mycoplasma hominis biology.organism_classification physiological processes Molecular biology Microbiology law.invention Complementation Plasmid law Escherichia Genetics biology.protein Recombinant DNA bacteria biological phenomena cell phenomena and immunity FtsZ Gene |
| Zdroj: | Russian Journal of Genetics. 39:249-255 |
| ISSN: | 1022-7954 |
| Popis: | A Mycoplasma hominis chromosomal fragment containing the full-length ftsZ gene was cloned and sequenced. Natural expression of this gene was demonstrated by reverse transcription–polymerase chain reaction (RT–PCR) with total RNA. TheM. hominis FtsZ protein was shown to differ substantially from its counterparts of two otherMycoplasma species, M. genitalium,and M. pneumoniae.The possibility of M. hominis ftsZ expression in Escherichia coliwas demonstrated with several bacterial strains. The M. hominis FtsZ protein was isolated from E. coli cells transformed with recombinant plasmids carrying the M. hominis ftsZ gene. Complementation between theE. coli and M. hominis FtsZ proteins was observed in transformants. |
| Databáze: | OpenAIRE |
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