| Autor: |
Christine Kongstad, Ripudaman Singh, Katarina Ravn, Lotte Hatt, Pinar Bor, Ida Vogel, Niels Uldbjerg |
| Rok vydání: |
2022 |
| Předmět: |
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| DOI: |
10.21203/rs.3.rs-1350735/v1 |
| Popis: |
Introduction Two approaches have been suggested for cell-based non-invasive prenatal testing (cbNIPT); A) enrichment of extravillous trophoblasts (EVTs) from maternal blood using antibodies targeting a combination of mesodermal and ectodermal markers and B) enrichment of EVTs from cervical swabs using antibodies targeting human leukocyte antigen G. Objective To investigate whether a well-established protocol for enrichment of EVTs from maternal blood sample can be used on cervical swabs. Methods A blood sample and a cervical swab from each of the ten women carrying male fetuses and undergoing first trimester termination of pregnancy were obtained. A previously published method, ‘trophoblast retrieval and isolation from the cervix’ was used for mucus dissolvement before enriching EVTs using antibodies against CD105 and CD141, as used for enrichment of EVTs from maternal blood. XY chromosome fluorescence in situ hybridization was performed to validate potential EVTs. Results No EVTs (XY cells) were detected in the cervical samples. Only maternal cells of epithelial origin were identified. However, the number of EVTs in blood samples varied from 1-9 EVTs per 30 mL.Conclusion A cbNIPT antibody-based protocol developed for enrichment of EVTs from maternal blood failed to enrich EVTs from the cervical canal of pregnant women in first trimester. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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