A combined feeding strategy for enhancing mycophenolic acid production by fed-batch fermentation in Penicillium brevicompactum
| Autor: | Dongzhi Wei, Bai Chaogang, Jian Zhang, Lihua Wang, Aiyou Sun, Rui Xu, Yuguo Dong |
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| Rok vydání: | 2015 |
| Předmět: |
Industrial equipment
biology Batch fermentation business.industry Substrate (chemistry) Penicillium brevicompactum Bioengineering Synthesis Phase Industrial fermentation biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Mycophenolic acid Biotechnology medicine Fermentation Food science business medicine.drug |
| Zdroj: | Process Biochemistry. 50:336-340 |
| ISSN: | 1359-5113 |
| Popis: | A combined feeding strategy for enhancing mycophenolic acid production by fed-batch fermentation in Penicillium brevicompactum was investigated. In a 7 L fermenter, the relevant parameters of CN-feeding strategy (F-CN), pH shift strategy (F-pH), and Met-feeding strategy (F-Met) were inspected and analyzed during the synthesis phase of MPA fermentation after 120 h. Compared to the fed-batch fermentation, the three strategies improved the MPA production to 2.23, 1.92, and 2.03 g/L, increased by 29.65, 11.63, and 18.02%, respectively. Based on the above results, a combined strategy (F-CPM) was designed. Under F-CPM strategy, the maximum dry cell weight (DCWmax) reached 45.77 g/L at 240 h, and the maximum MPA production (Pmax) reached a higher level of 2.68 g/L, increased by 55.81%. The combined feeding strategy can effectively reduce the impact of substrate inhibition and improve MPA productivity. Thus, our work offered a new idea for industrialization of MPA fermentation with conventional industrial equipment. |
| Databáze: | OpenAIRE |
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