Dynamin-dependent vesicle twist at the final stage of clathrin-mediated endocytosis

Autor: Xiaodong Cheng, Gufeng Wang, Seth L. Filbrun, Ning Fang, Bin Dong, Teng-Xiang Huang, Yan Gu, Kuangcai Chen, Meek Yang, Yong Myoung
Rok vydání: 2021
Předmět:
Zdroj: Nature Cell Biology. 23:859-869
ISSN: 1476-4679
1465-7392
Popis: Dynamin has an important role in clathrin-mediated endocytosis by cutting the neck of nascent vesicles from the cell membrane. Here, using gold nanorods as cargos to image dynamin action during live clathrin-mediated endocytosis, we show that, near the peak of dynamin accumulation, the cargo-containing vesicles always exhibit abrupt, right-handed rotations that finish in a short time (~0.28 s). The large and quick twist, herein named the super twist, is the result of the coordinated dynamin helix action upon GTP hydrolysis. After the super twist, the rotational freedom of the vesicle increases substantially, accompanied by simultaneous or delayed translational movement, indicating that it detaches from the cell membrane. These observations suggest that dynamin-mediated scission involves a large torque generated by the coordinated actions of multiple dynamins in the helix, which is the main driving force for vesicle scission. Using gold nanorods and 3D-SPORT to track dynamin-induced fission in live cells, Cheng et al. show that endocytic vesicles undergo a rapid, large rotation before fission, which requires dynamin’s GTPase activity.
Databáze: OpenAIRE
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