Simultaneous Evaluations of GSH Levels, SOD Activity and the Genotoxic Damage in Mice Treated with Chromium(VI) and Catechins of Green Tea [(+)-Catechin and (-)-Epigallocatechin-3-Gallate]
| Autor: | Mario Altamirano-Lozano, Gabriela Serrano-Reyes, María del Carmen García-Rodríguez, Tonancy Nicolás-Méndez |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
chemistry.chemical_classification
Reactive oxygen species Antioxidant DNA damage Stereochemistry medicine.medical_treatment Cell food and beverages Catechin Glutathione Pharmacology Biochemistry chemistry.chemical_compound medicine.anatomical_structure chemistry Apoptosis Physiology (medical) medicine Micronucleus |
| Zdroj: | Free Radical Biology and Medicine. 100:S106 |
| ISSN: | 0891-5849 |
| DOI: | 10.1016/j.freeradbiomed.2016.10.271 |
| Popis: | The compounds of Cr(VI) generates reactive oxygen species (ROS) during its reduction to Cr(III) leading to DNA damage. The green tea polyphenols can protect against the genotoxic damage induced by Cr(VI) by reducing frequencies of micronucleus MN[1]. In this study, the effect of catechins of green tea [(+)-catechin and epigallocatechin-3-gallate (EGCG)] on glutathione (GSH) levels and superoxidodismutasa (SOD) activity was evaluated simultaneously with the analysis of MN and apoptosis. Groups of five Hsd-ICR mice were divided and treated as follows: i) only vehicle; ii) (+)-catechin (20 and 40 mg/kg) or EGCG (10 mg/kg) by gavage; iii) CrO3 (20 mg/kg) intraperitoneally; and iv) (+)-catechin or EGCG in addition to CrO3. Evaluations were performed at 0, 24, 48 and 72 h after treatments. No significant changes in MN frequencies were observed when the catechins of green tea were administrated alone. However, the treatment with CrO3 showed an increase in MN, apoptosis and GSH levels, but the activity of SOD decreased. The treatment with (+)-catechin prior to administration of CrO3 showed no effects on MN and apoptosis. However, treatment with EGCG possibly presents both effects; prooxidant and antioxidant because the MN was increased at 24 h, but it was reduced at 48 h when EGCG was administrated prior to CrO3. Additionally, GSH levels decreased and SOD activity is recovered. The increase of apoptotic cells suggests that this may play a role in the elimination of micronucleated cells. Financial support was obtained from DGAPA-UNAM PAPIIT IN219216; IN217712; PAEP-2016; CONACYT 703847/584686. Reference [1] Maria del Carmen Garcia-Rodriguez, et al. 2013. Oxid Med Cell Longev, vol. 2013, Article ID 486419, 9 pag. doi:10.1155/2013/486419. |
| Databáze: | OpenAIRE |
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