In vivo MRI of olfactory ensheathing cell grafts and regenerating axons in transplant mediated repair of the adult rat optic nerve
| Autor: | Martin Berry, Ioanna Sandvig, Øystein Olsen, Susan C. Barnett, Axel Sandvig, Olav Haraldseth, Kåre E. Tvedt, Thomas C. P. Sardella, Linh Hoang, Christian Brekken, Marte Thuen |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.diagnostic_test Regeneration (biology) Cell Magnetic resonance imaging Biology medicine.anatomical_structure Retinal ganglion cell In vivo medicine Optic nerve Molecular Medicine Radiology Nuclear Medicine and imaging Olfactory ensheathing glia Remyelination Spectroscopy |
| Zdroj: | NMR in Biomedicine. 25:620-631 |
| ISSN: | 0952-3480 |
| Popis: | The purpose of the present study was to use magnetic resonance imaging (MRI) as a tool for monitoring transplant-mediated repair of the adult rat visual pathway. We labelled rat olfactory ensheathing cells (OECs) using micron-sized particles of iron oxide (MPIO) and transplanted them by: i) intravitreal injection (ivit) and ii) intra-optic nerve (ON) injection (iON) in adult rats with ON crush (ONC) injury. We applied T(2)-weighted MRI and manganese-enhanced MRI (MEMRI) to visualise transplanted cells and ON axons at specific times after injury and cell engraftment. Our findings demonstrate that ivit MPIO-labelled OECs are unequivocally detected by T(2)-weighted MRI in vivo and that the T(1)-weighted 3D FLASH sequence applied for MEMRI facilitates simultaneous visualisation of Mn(2+-) enhanced regenerating retinal ganglion cell (RGC) axons and MPIO-labelled OEC grafts. Furthermore, analysis of MRI data and ultrastructural findings supports the hypothesis that iON OEC transplants mediate regeneration and remyelination of RGC axons post injury. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text