Development and bioassay ofCry1Ac-transgenic eggplant (Solanum melongenaL.) resistant to shoot and fruit borer
| Autor: | S. Satpathy, Mohit Kumar Rai, J. K. Pal, Manpreet Singh, Sanjeet Kumar, D. V. Singh |
|---|---|
| Rok vydání: | 2009 |
| Předmět: | |
| Zdroj: | The Journal of Horticultural Science and Biotechnology. 84:434-438 |
| ISSN: | 2380-4084 1462-0316 |
| DOI: | 10.1080/14620316.2009.11512545 |
| Popis: | SummaryEggplant (Solanum melongena L.) is an important fruit vegetable, commercially cultivated in the tropics and subtropics. However, the most serious constraint on eggplant production is damage caused by eggplant shoot and fruit borer (ESFB). The development of resistant transgenic plants, using an insecticidal crystal protein gene, is an available crop protection option. A Cry1Ac gene obtained from the National Research Center for Plant Biotechnology (NRCPB), New Delhi, India, was transformed via Agrobacterium-mediated gene transfer into an improved inbred line of eggplant (IVBL-9). Hypocotyls proved to be the most effective and suitable explants, with a transformation frequency of 17.3% and 2.9 shoots per explant. PCR and Southern blot analyses confirmed the presence of a single copy insertion of the Cry1Ac gene in seven independent transgenic plants. The insertion of a single copy of the gene was also confirmed by segregation analysis of T1 seed from T0 plants. ELISA analyses revealed the presence o... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|