Determination of oleanolic acid and ursolic acid in Oldenlandia diffusa and its substitute using high performance liquid chromatography
| Autor: | Zhi-Hong Jiang, Zhitao Liang, Zhongzhen Zhao, David Wang Fun Fong |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pharmacology
Oldenlandia diffusa 0303 health sciences Chromatography 030309 nutrition & dietetics Systematic survey 010401 analytical chemistry Extraction (chemistry) Biology 01 natural sciences High-performance liquid chromatography 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound Ursolic acid chemistry Evaluation methods Medicinal plants Oleanolic acid Food Science |
| Zdroj: | Journal of Food and Drug Analysis. 17 |
| ISSN: | 2224-6614 |
| DOI: | 10.38212/2224-6614.2291 |
| Popis: | An improved quality evaluation method for Oldenlandia diffusa (Willd.) Roxb. and its substitute was established by a well-defined high performance liquid chromatographic (HPLC) method. Firstly, a complete separation of oleanolic acid and ursolic acid was successfully achieved by optimizing the mobile phase systems based on previous reports. The extraction method was optimized and the less toxic solvent was selected for extraction. The systematic survey of herbal markets has shown that O. corymbosa (L.) Lam are widely available and used indiscriminately as O. diffusa. Twenty-seven samples of O. diffusa and seventeen samples of O. corymbosa were analyzed. The results showed that the contents of oleanolic acid and ursolic acid in O. diffusa were generally lower by almost two times than those in O. corymbosa. Moreover, the contents of oleanolic acid and ursolic acid between the fresh and dry samples were compared. The introduction of quality evaluation method could be useful for the species identity and quality evaluation of O. diffusa and its substitute. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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