SHORT COMMUNICATION: Lineage study of degenerating photoreceptor cells in the rd mouse retina
| Autor: | Janet C. Blanks, Kathryn A. Rich, Susan Y. Schmidt, Christine Spee, Ernesto Barron |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Retina
Lineage (genetic) genetic structures Cell division biology lac operon Anatomy biology.organism_classification eye diseases Sensory Systems Cell biology Cellular and Molecular Neuroscience Ophthalmology Vibratome medicine.anatomical_structure Retrovirus Neuroblast Mouse Retina medicine sense organs |
| Zdroj: | Current Eye Research. 16:733-737 |
| ISSN: | 1460-2202 0271-3683 |
| DOI: | 10.1076/ceyr.16.7.733.5067 |
| Popis: | Purpose. A retroviral marker was used to label daughter cells arising from individual neuroblasts in the rd mouse retina, in order to investigate the hypothesis that a clonal relationship exists among degenerating photoreceptor cells. METHODS. On the day of birth, a single injection of retrovirus with a lac Z (s-galactosidase) reporter construct was injected into the retina in the vicinity of the subretinal space. Descendants of single neuroblasts were identified histochemically by examining the retinas at P14 (postnatal day 14). Light and electron microscopic studies were used to identify the retrovirally-induced marker s-D-galactosidase using Bluo-gal dye. Double-labeling of degenerating cone cells was accomplished by taking 100 mm vibratome sections of retrovirally-injected eyes and using either FITC-PNA or HRP-PNA to visualize clusters of degenerating cones as well as Bluo-gal labeled clones of photoreceptor cells on the same tissue section. RESULTS. A relatively large number of clones of primarily ph... |
| Databáze: | OpenAIRE |
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