An Update on Aptamer-Based Multiplex System Approaches for the Detection of Common Foodborne Pathogens

Autor: Omar Mukama, Jean Paul Sinumvayo, Muhammad Shamoon, Muhammad Shoaib, Henriette Mushimiyimana, Waseem Safdar, Leo Bemena, Peter Rwibasira, Samson Mugisha, Zhouping Wang
Rok vydání: 2017
Předmět:
Zdroj: Food Analytical Methods. 10:2549-2565
ISSN: 1936-976X
1936-9751
DOI: 10.1007/s12161-017-0814-5
Popis: Foodborne ailments constitute a public health challenge and pose an incredible economic burden in healthcare system around the globe. This dilemma has urged authorities and other entities working in field of food quality control and supply chain to play a pivotal role in ensuring food safety. Analytical strategies have been developed using numerous systematic evolution of ligands by exponential enrichment (SELEX) methods to assure food safety. High-affinity and high-sensitivity ssDNA and RNA aptamers against pathogens have emerged as a novel strategy, as compared to the more resource-demanding and complicated biochemical test-based approaches. Thus, this review aims to focus on some methods used in the selection of specific bare, modified, and conjugated aptamers and on the further analysis of selected aptamers using flow cytometer or post-SELEX modifications for enhanced detection of frequently diagnosed foodborne bacteria such as Bacillus sp., Campylobacter jejuni, Escherichia sp., Salmonella sp., Staphylococcus aureus, Shigella sp., Listeria monocytogenes, and Streptococcus pyogenes and/or targeting their cell components towards attaining fast, sensitive, and selective methods for the detection of pathogens in food(s) or other sources.
Databáze: OpenAIRE
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