Use of porous glass fiber as a support for biocatalyst immobilization

Autor:	George T. Tsao, Norman B. Jansen, Fidel Toldrá
Rok vydání:	1986
Předmět:	Pressure drop Materials science Chromatography Glass fiber Bioengineering General Medicine Maltose Porous glass Applied Microbiology and Biotechnology chemistry.chemical_compound chemistry Chemical engineering Covalent bond Coupling (piping) Tube (fluid conveyance) Fiber Biotechnology
Zdroj:	Biotechnology Letters. 8:785-790
ISSN:	1573-6776 0141-5492
Popis:	Porous glass fiber has a very high surface area and good mechanical properties that make it an excellent support for biocatalyst immobilization. By packing aligned glass fibers in a tubular reactor such that the fibers are all parallel to the axis of the tube, the resulting pressure drop is considerably smaller than for a similar bed of packed beads. The utility of this support was demonstrated by immobilizing α-glucoamylase by silane-glutaraldehyde coupling, and measuring its activity toward converting maltose to glucose. Using optimized immobilization conditions, an enzyme loading of 1.5 mg protein perm 2 surface area was obtained, with an activity of 370 units/g glass at 50°C. The half-life of the immobilized glucoamylase was more than twice as long as that of the free enzyme.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a4e0b68ac3d1c317576b2d35ca9ac486 https://doi.org/10.1007/bf01020823 Zobrazit plný text záznamu Full text from SpringerLink