BioArena: An unlimited possibility of biochemical interactions in the adsorbent layer after chromatographic separation

Autor: Ernö Tyihák, Zsuzsa Király-Véghely, György Kátay, Emil Mincsovics, Péter G. Ott, Ágnes M. Móricz
Rok vydání: 2008
Předmět:
Zdroj: Journal of Planar Chromatography – Modern TLC. 21:15-20
ISSN: 1789-0993
0933-4173
DOI: 10.1556/jpc.21.2008.1.2
Popis: The BioArena system, which integrates the modern technique and biological results of bioautography with layer liquid chromatography is especially suitable for investigating biochemical interactions. Formaldehyde (HCHO) and its reaction products play a crucial role in the antibiotic activity of trans -resveratrol and other molecules — when HCHO-capturing molecules are used in culture media the antimicrobial activity of antibiotic-like compounds decreases substantially. HCHO and hydrogen peroxide are present as normal endogenous compounds in cells, so there is a possibility of interaction in which singlet oxygen ( 1 O 2 ) and excited HCHO can be formed. The 1 O 2 can oxidize water molecules and so H 2 O 3 can be formed, from which, by disproportion, among other reactions, ozone (O 3 ) also can be formed in the chromatographic spots. Elimination of HCHO and/or O 3 from the spots results in a decrease in the antiproliferative effect.
Databáze: OpenAIRE
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