Profiling of Serum Extracellular Vesicles Reveals miRNA-4525 as a Potential Biomarker for Advanced Renal Cell Carcinoma
| Autor: | Masafumi Ito, Kosuke Mizutani, Yuka Muramatsu-Maekawa, Yuri Miura, Daiki Kato, Kyojiro Kawakami, Yasunori Fujita, Keita Nakane, Tomohiro Tsuchiya, Taku Kato, Takuya Koie, Manabu Takai |
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| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
Microarray analysis techniques Mucin Extracellular vesicle Transfection Biology urologic and male genital diseases medicine.disease Biochemistry female genital diseases and pregnancy complications Biomarker (cell) Renal cell carcinoma microRNA Genetics Cancer research medicine Alpha-fetoprotein Molecular Biology |
| Zdroj: | Cancer Genomics - Proteomics. 18:253-259 |
| ISSN: | 1790-6245 1109-6535 |
| Popis: | Aim To identify novel diagnostic markers for renal cell carcinoma (RCC), we analyzed miRNAs in serum extracellular vesicles (EVs). Materials and methods EVs were purified from serum of healthy controls and patients with localized and advanced RCC using T-cell immunoglobulin domain and mucin domain-containing protein 4 conjugated to magnetic beads. miRNA profiling of EVs was conducted by microarray analysis. miRNA expression was examined by quantitative reverse transcription-polymerase chain reaction. Lastly, proteomic analysis of RCC cells transfected with a miRNA inhibitor was performed to identify its potential targets. Results Microarray analysis revealed that nine miRNAs were increased by more than 1.5-fold in EVs from patients with RCC. Among them, miRNA-4525 was significantly elevated; miRNA-4525 expression was higher in RCC tissue than in the adjacent normal tissue. Proteomic analysis identified alpha fetoprotein and albumin as its potential targets. Conclusion These findings suggest the potential of miRNA-4525 in serum EVs as a novel biomarker for advanced RCC. |
| Databáze: | OpenAIRE |
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