Effect of low level laser therapy in a model of pulmonary idiopathic fibrosis
| Autor: | Nicole Cristine Rigonato-Oliveira, Gabriel de Oliveira Isac Moraes, Luana Beatriz Vitoretti, Jefferson Comin Jonco Aquino-Junior, Ana Paula Ligeiro-Oliveira, Clariana Soares, Rodolfo de Paula Vieira, Auriléia Aparecida de Brito |
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| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.diagnostic_test business.industry medicine.medical_treatment respiratory system medicine.disease Bleomycin Pathophysiology respiratory tract diseases Interleukin 10 Idiopathic pulmonary fibrosis chemistry.chemical_compound Bronchoalveolar lavage chemistry Fibrosis medicine Tumor necrosis factor alpha business Low level laser therapy |
| Zdroj: | 3.3 Mechanisms of Lung Injury and Repair. |
| DOI: | 10.1183/13993003.congress-2015.pa945 |
| Popis: | Idiopathic pulmonary fibrosis (IPF) is an interstitial disease with genetic and environmental risk factors. Experimental models attempt to clarify its pathophysiology and the discovery of new therapies. Therapy with low level laser therapy (LLL) is a possible use in the treatment of pulmonary diseases. Therefore this study investigated the effects of LLL on experimental model of bleomycin-induced IPF. C57BL/6 mice were divided into 3 groups: control, fibrosis and fibrosis+LLL. The animals received a single administration of bleomycin sulfate (1,5Ui/kg it). After 14 days animals started LLL (diodo laser, 660nm, 30mW e 3J / cm²) for 7 days (1x/day). 24 hours after the last irradiation, the animals were euthanized. Collected the bronchoalveolar lavage (BAL) and lungs. Three animals were used for the primary culture of pneumocytes type II and fibroblasts, stimulated with bleomycin with or without irradiated LLL. Evaluated the levels of IL1 β , IL6, TNFα, TGF β , IL10, IL17, IFNγ and KC in the supernatants of cells and BAL by ELISA. We also made collagen analysis airway, stained with Picrocirius. Immunohistochemistry was performed for quantification of PCNA and caspase 3. The results indicate that LLL reduced pulmonary inflammation, by reduction cell numbers in the BAL (p |
| Databáze: | OpenAIRE |
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