Determination of the breakpoint of the common α-thalassaemia deletion in Filipinos in Hawaii

Autor:	John A. Hunt, Lara K Lee, Y. Edward Hsia, Timothy A. Donlon
Rok vydání:	1999
Předmět:	Genetics Restriction map Base pair hemic and lymphatic diseases Crossover Breakpoint Multiplex Hematology Globin Biology Gene Molecular biology DNA sequencing
Zdroj:	British Journal of Haematology. 104:284-287
ISSN:	0007-1048
DOI:	10.1046/j.1365-2141.1999.01175.x
Popis:	The region of the crossover causing the Filipino type of α thalassaemia has been determined by examining similarity between the regions which had been indicated as involved in the crossover points by restriction mapping, using the published α-globin region DNA sequence. The crossover point was found in 21 base pairs between two Alu sequences using PCR primers flanking these Alu sequences. A simple PCR multiplex assay has been devised to detect heterozygotes and homozygotes.
Databáze:	OpenAIRE
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